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By T.K. Ghose ,A. Fiechter

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Extra resources for Advances in Biochemical Engineering, Volume 001

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Vol. 1 34 S. Aiba and M. Nagatani ~Pulley iley / Inlet and ~ Outlet ~ II~ I~ Licjht Ammeter @ Photo- Sampler micrograph l l I ' I ! C. Voltage Stabilizer Fig. la. Optical arrangement to observe continuously flocculation and deflocculation ! , ~ CdSelement ~ I~l ~I~ /S~ilicon- rubber packing . jLid of vessel T Li~ (acrylic resin) ~~Glass plate Jbberpacking resin) ( Fig. 1c. Light-recipient, unit = mm DiF Lamp zmpere) Fig. 1d. Sampling device, unit = mm Fig. 1b. Light-source, unit = mm Separation of Cells from Culture Media 35 Assuming that a definite resistance (500 ohm) in the circuit is small enough, the resistance, R of the CdS element is correlated with the recorder reading, V (millivolt) by the following equation.

If a solid line is assumed as shown in Fig. 4, the floc size, ffl could be assessed from the observed value of -logT2. Taking the fact that the cell suspension is dilute enough for granted, a relationship between the suspension turbidity, - l o g T and the cell number concentration, N is: - log T i = log(I0/I1) = kl (7r/4) d~ N1, (3) - log T2 = log(Io/12) = k 2(rr/4) ~f N 2 . 0 Ion odded , Symbol ® Co ~(2~4 ) xlO-tJ~n- A [] AI e , 4 . C / /, I 2 4 f 6 f 8 I 10 20 I 40 60 dp Fig. 4. -log T1/-log T2 vs.

A. I. E. Journal 11, 815 (1965). Bartholomew, W. : Advance in Appl. Microbiol. 2, 289 (1960). : Biotechnol. Bioeng. 8, 25 (1966). 30 H. Taguchi: The Nature of Fermentation Fluids Chain, E. : Biotechnol. Bioeng 8, 595 (1966). Darlington, W. : Bioteehnol. Bioeng. 6, 241 (1964). Deindoerfer, F. , Wise, J. : Advance in Appl. MicrobioL 2, 265 (1960). Erickson, L. , Humphrey, A. : Biotechnol. Bioeng. 11, 449 (1969). - - Biotechnol. Bioeng. 11, 467 (1969). - - Biotechnol. Bioeng 11, 489 (1969). Fondy, P.

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