Download Antiviral Resistance in Plants: Methods and Protocols by John A. Lindbo (auth.), John M. Watson, Ming-Bo Wang (eds.) PDF

By John A. Lindbo (auth.), John M. Watson, Ming-Bo Wang (eds.)

Studies with regards to pathogen-mediated virus resistance in vegetation have been instrumental in supplying a few of the historic observations which finally resulted in the important discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has on account that revolutionized learn on plant-virus interactions. In Antiviral Resistance in vegetation: tools and Protocols, specialist researchers within the box element the various equipment that are now regularly occurring to review the phenomenon of RNA silencing when it comes to viral infections of crops. those comprise tools and strategies for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs similar to small interfering RNAs (siRNAs) and microRNAs (miRNAs) in addition to the research and manipulation of virus-induced gene silencing (VIGS) in either monocotyledonous and dicotyledenous crops and using hairpin RNA (hpRNA) transgenes. Written within the hugely winning Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, without problems reproducible laboratory protocols, and key pointers on troubleshooting and warding off identified pitfalls.

Authoritative and sensible, Antiviral Resistance in vegetation: tools and Protocols seeks to help scientists within the additional research of this crucially vital botanical trait.

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However, the biological significance of this possible DCL1mediated negative control of antiviral silencing remains unclear. In addition to DCLs and RDRs, a dsRNA-binding protein, DRB4, and AGO1 and AGO7, have been shown to participate in 28 C. Agius et al. the DCL4-initiated antiviral silencing pathway. DRB4 has been previously shown to assist DCL4 in endogenous ta-siRNA biogenesis. Consistent with its functional interaction with DCL4, a recent study showed that the accumulation of 21-nucleotide siRNAs from tomato spotted wilt virus was abolished in a drb4 mutant (8).

To account for this strand bias, Molnár and colleagues (93), based on their analysis of siRNA distribution from Cymbidium ringspot tombusvirus, proposed a model where certain regions of the viral ssRNAs may form partially double-stranded secondary structures that are processed by a DCL into siRNAs. This local base-pairing model has also been suggested to account for siRNA production from the translational leader sequence of the cauliflower mosaic DNA virus that forms extensive secondary structures (94).

Genes Dev 19:2164–2175 5. Xie Z, Allen E, Wilken A, Carrington JC (2005) DICER-LIKE 4 functions in trans-acting small interfering RNA biogenesis and vegetative phase change in Arabidopsis thaliana. Proc Natl Acad Sci U S A 102: 12984–12989 6. Rajagopalan R, Vaucheret H, Trejo J, Bartel DP (2006) A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana. Genes Dev 20:3407–3425 7. Margis R, Fusaro AF, Smith NA, Curtin SJ, Watson JM, Finnegan EJ, Waterhouse PM (2006) The evolution and diversification of Dicers in plants.

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