Download Capillary Electrophoresis of Biomolecules: Methods and by Chenchen Wang, Xueping Fang, Cheng S. Lee (auth.), Nicola PDF

By Chenchen Wang, Xueping Fang, Cheng S. Lee (auth.), Nicola Volpi, Francesca Maccari (eds.)

Capillary electrophoresis (CE) is a comparatively new separation method compatible for dealing with small quantities of pattern vitally important in bioanalytical study and in a number of medical, diagnostic, genetic, and forensic purposes. In Capillary Electrophoresis of Biomolecules: equipment and Protocols, professional researchers within the box offer key options to enquire CE concentrating on easy and complicated carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids. in addition to functional approaches, reports discussing CE functions concerning bio(macro)molecules also are integrated. Written within the hugely profitable Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, without problems reproducible laboratory protocols, and key tips about troubleshooting and averting identified pitfalls.

Authoritative and functional, Capillary Electrophoresis of Biomolecules: equipment and Protocols presents the reader with the newest holiday throughs and enhancements in CE and CE concepts utilized to numerous sessions of bio(macro)molecules.

Show description

Read or Download Capillary Electrophoresis of Biomolecules: Methods and Protocols PDF

Best biology books

Aestivation: Molecular and Physiological Aspects

A number of animal species stay in environments characterised by means of a seasonal relief within the availability of water, which frequently yet now not regularly happens while temperatures are optimum. for plenty of such animals, survival through the hardest season calls for spending lengthy sessions of time in a slightly inactive nation referred to as aestivation.

Biology of Inorganic Nitrogen and Sulfur

Nitrogen and sulfur compounds are regularly synthetized, degraded and switched over into other kinds in nature. there are numerous similarities within the precept difficulties and easy mechanisms of the biology of inorganic nitrogen and sulfur. Many info should not but understood and accordingly are the topic of energetic research internationally.

Additional resources for Capillary Electrophoresis of Biomolecules: Methods and Protocols

Example text

Humana Press, New York, pp 23–52 3. Evangelista RA, Liu MS, Chen FTA (1995) Characterization of 9-aminopyrene-1,4,6trisulfonate-derivatized sugars by capillary electrophoresis with laser-induced fluorescence detection. Anal Chem 67:2239–2245 4. Lv Z, Sun Y, Wang Y, Jiang T, Yu G (2005) Ultrasensitive capillary electrophoresis of oligoguluronates with laser-induced fluorescence detection. Chromatographia 61:615–618 5. García Moreno MDV, Castro Mejías R, Natera Marín R, García Barroso C (2002) Analysis of sugar acids by capillary electrophoresis with indirect UV detection.

Kazarian et al. used a low-pH sample (formic acid) and high-pH electrolyte (borate) to improve the sensitivity of carbohydrates modified with novel fluorescein reagent (40–42). This strategy was chosen because the derivatization reaction occurs at low pH and can be directly injected into the capillary, thereby avoiding the dilution step that is normally required. A 515fold improvement in sensitivity was obtained (Fig. 8) and was later implemented in microchips. One of the advantages of stacking with a dynamic pH junction is that it is tolerant of salt and is therefore compatible with highly saline solutions.

They applied their method to analysis of a proteinase K digest of thyroid-stimulating hormone (TSH). This glycoprotein contains several sulfonylated N-glycans including the 4-sulfonylated-LDn type that contains sulfonylated GalNAc in the antennae. The CE/MS data, acquired using negative-ion electrospray, showed abundant ions, demonstrating effective enrichment, from sulfonylated glycopeptides. The authors also showed that the glycopeptides could be analyzed using positive-ion electrospray MS by including a basic peptide of sequence KKK in the sheath liquid.

Download PDF sample

Rated 4.43 of 5 – based on 36 votes