Download Cell and Tissue Culture Models in Dermatological Research by M. Bachmann, W. E. G. Müller (auth.), Dr. August Bernd, PDF

By M. Bachmann, W. E. G. Müller (auth.), Dr. August Bernd, Professor Dr. Jürgen Bereiter-Hahn, Priv.-Doz. Dr. Frank Hevert, Professor Dr. H. Holzmann (eds.)

In this quantity the influence of phone tradition types on dermatological learn is mentioned via scientists from drugs, body structure, biology, pharmacology,and pharmacy. The ebook contains the next themes: orthology and pathology of the outside, differentiation of keratinocytes and fibroblasts, difficulties of the cultivation of melanocytes, biochemistry of pigment metabolism, types for wound therapeutic and tissue renewal, fibroblast functionality and metabolism of collagen, versions for the research of getting older in addition to versions for pharmacological and toxicologial checks. therefore, a large arch is spanned, from uncomplicated issues of cultivation and characterization of phone cultures to examples of software. Dermatologists in addition to telephone biologists will reap the benefits of this publication.

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3 u.. 4 ..... 1 o Fig. Ie 2000 4000 6000 Molecules [ltiJ-\2S(OH~D.! I cell % of control 120 110 100 90 80 70 60 50 40 30 20 10 0 2 3 4 Fig. 2. 7) at concentrations of 10 nrn/I (1 % FCS, 5% CO2). 1, Control medium; 2, IL la; 3, IL 1/3; 4, TNFa (Fig. 8%), while TNF-a distinctively reduced the number of cells in the two dividing cell cycle compartments. 4%). Cytokeratin 1110 and Ki-67 expression was reduced in TNF-a- and upregulated in IL-la-stimulated HaCaT keratinocytes (Fig. 4). 41 Modulation of 1,25-Dihydroxy (VDR) Expression in HaCaT Keratinocytes 253 .

J Invest Dermatol 95: 2S-4S Influence of Human Sera on the Adherence of Candida albicans to Buccal and Intestinal Cells in Culture H. Hanel, M. Buslau, F. Dey, A. Bernd, and J. Bereiter-Hahn Abstract We investigated whether serum of healthy persons or seborrhoic patients influences the adhesion of Candida albicans isolated from healthy or seborrhoic patients to human intestine and buccal cells of healthy or seborrhoic patients. Serum from healthy persons inhibited the adhesion to intestine cells less than serum of seborrhoic patients did.

Op. S (l) 100 ~ ;0 ·s :>, t: 50 0 0 • \l 5% Human AS pool serum Control collective Psoriasis collective 10% 20% 40% Fig. 4. Concentration dependence of mitogenic stimulation of human embryonic skin fibroblasts by psoriasis and control sera. The incorporation of 3H-thymidine is given in dpm/mg protein. l 0 Fig. 5. Effect of psoriasis serum on the DNA synthesis of human keratinocytes in synchronized and nonsynchronized cultures. The cells were incubated with human AB pool serum, pooled serum of the normal collective, and pooled serum of the psorias is collective at 37 DC, as described under Methods.

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