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By Professor Dr. Wilhelm Möller (auth.)

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Extra info for Circumventriculare Organe in der Gewebekultur / Circumventricular Organs in Cell Culture

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Vergr. a und c 400 x, b 250 x 50 Striingen verdichtet (vgl. Abb. 17). Von solchen, im histochemischen Priiparat glykogenfreien Zellen fiihren verschiedene Dbergangsformen zu Zellelementen, die nur noch uber ihre Fortsatze untereinander in Kontakt stehen oder vollig isoliert sind (Abb. 20). Diese Fortsiitze, die mehr oder weniger stark ausgepriigte undulierende Cytoplasmamembranen besitzen, lassen sich durch Versilberung nach Bodian/Ziesmer darstellen. Diese Darstellbarkeit beruht auf dem Vorhandensein fibrillarer Strukturen, die bereits in friiheren glykogenreichen Stadit'm durch Kombinationsfarbung BodianPAS nachgewiesen·werden konnen.

Deckglaskulturen wurden in erster Linie mit 2 % Glutaraldehyd fixiert. Einige Kulturen wurden mit 4 %igem Formol, nach Bouin sowie nach Rossmann (1940) behandelt. Ais Farbungen wurden die PAS- und Bleitetraacetat/Schiff-Verfahren (Shimuzu und Kumamoto, 1952) mit Diastasekontrolle, zum Teil in Kombination 1. mit der Versilberung nach Bodian/Ziesmer (Ziesmer, 1952) oder 2. mit Sudan schwarz bzw. Sudangrtin in Propylenglykoll (Chiffelle und Putt, 1951) verwendet. , 1967) durchgefiihrt. Lm dick geschnitten.

D) Dieselbe Kultur wie in c. Lm, Thionin-Methylenblau. Nur die perinukleare und die auBerste Cytopiasmaregion sind dargestellt. An der Oberseite der Kultur 2 Zellen mit mehreren Glykogenkompartimenten und Lipidvakuolen. Vergr. c und d 540 x Fig. 13a-d. Glycogen body cells cultured with high cell density, 18 day chick embryo. (a) 27 days in culture, glutaraldehyde, PAS x 35. Netlike aggregation of glycogen body cells, which lie in severa I layers. (b) 4 days in culture, glutaraldehyde, PAS, x 250.

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