By Christina Thompson
During this related to, compassionate memoir, Christina Thompson tells the tale of her romance and eventual marriage to a Maori guy, interspersing it with a story background of the cultural collision among Westerners and the Maoris of latest Zealand. regardless of their major changes, Thompson and her husband, Seven, proportion an identical experience of experience and a willingness to go away from the customs in their households and forge a lifestyles jointly on their lonesome. Thompson explores cultural displacement in the course of the a while and the interesting heritage of Europeans within the South Pacific, starting with Abel Tasman's discovery of recent Zealand in 1642. but at its middle, this is often the tale of 2 those that meet, fall in love, and are endlessly replaced.
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Extra info for Come on Shore and We Will Kill and Eat You All: A New Zealand Story
Cell culture medium: SAGM™ BulletKit®: Small Airway Epithelial Growth Medium (Lonza) with 1% Pen/Strep and 1% fetal calf serum (FCS). 2. , Transwell® or Snapwell® permeable filters (Corning Costar, Bodenheim, Germany) or Lab-Tek chamber slides (Nunc, Langenselbold, Germany)]. 3. Epithelial Voltohmmeter (EVOM, World Precision Instruments, Berlin, Germany) with chopstick electrodes. 4. Incubator (5% CO2, 95% relative humidity, 37°C). 3 Human Alveolar Epithelial Cells 35 Fig. 1. Schematic view of the PADDOCC system to aerosolize and deposit a dry powder aerosol realistically onto air–liquid interface grown lung epithelial cells (for detailed description, see ref.
Williams, M. C. (2003) Alveolar Type I Cells: Molecular Phenotype and Development, in Annual Review of Physiology, pp 669–695. 3. Fehrenbach, H. (2001) Alveolar epithelial type II cell: Defender of the alveolus revisited, Respiratory Research 2, 33–46. 4. Grainger, C. , Greenwell, L. , Lockley, D. , Martin, G. , and Forbes, B. (2006) Culture of Calu-3 cells at the air interface provides a representative model of the airway epithelial barrier, Pharmaceutical Research 23, 1482– 1490. 5. , and Ehrhardt, C.
Autoclaved dissection kit with curved scissors and forceps. 18. McIlwain® tissue chopper. 19. Sterile cell strainers of nylon (40- and 100-μm pores, Becton Dickinson) and gauze mesh (see Note 4). 20. Centrifuge with swing-out rotor. 21. Water bath with shaking function. 22. Neubauer hemocytometer. 23. Incubator (5% CO2, 95% relative humidity, 37°C). 2. Cultivation and Differentiation 1. Cell culture medium: SAGM™ BulletKit®: Small Airway Epithelial Growth Medium (Lonza) with 1% Pen/Strep and 1% fetal calf serum (FCS).