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SENKOWSKi 6. 2 Phase S o l u b i l i t y Phase s o l u b i l i t y a n a l y s i s i s c a r r i e d o u t u s i n g i s o p r o p a n o l as t h e s o l v e n t . A t y p i c a l example i s shown i n F i g u r e 8 which a l s o l i s t s t h e c o n d i t i o n s u n d e r which t h e analysis was carried out20. 3 Chromatographic A n a l y s i s Chromatographic a n a l y s i s may b e u s e d t o assess t h e s t a b i l i t y and p u r i t y of c h l o r d i a z e p o x i d e . 31 Thin-Layer Chromatographic A n a l y s i s The f o l l o w i n g TLC p r o c e d u r e i s u s e f u l f o r s e p a r a t i n g c h l o r d i a z e p o x i d e from p o s s i b l e h y d r o l y t i c p r o Using s i l i c a g e l G p l a t e s and e t h y l a c e t a t e ducts2'.

MICHAELIS, AND B. 2 . 1%) Additional TLC systems appearing in the literature are listed in Table IV. 32 Column Chromatographic Analysis The analytical scale liquid-solid chromatographic separation of chlordiazepoxide has been reported by Scott and Bommer in their study of the separation of several benzodiazepines from each other and from biological media27. The important advantages indicated for this method of analysis are the mild operating conditions which enable collection of the compounds and also the simple sample preparation which does not require derivatization or hydrolysis of the sample.

2 3 2 . I % EQUILIBRATION: L O H R S AT 2 5 % EXTRAPOLATED SOLUBILITY : 2 . 5 3 m g l g SOLVENT 0 V z 0 I3 J 0 v) 0 1 I 20 40 I 60 I 80 SYSTEM COMPOSITION: mg OF SAMPLE PER g OF SOLVENT 49 I' A. MacDONALD, A. F. MICHAELIS. AND B. 2 . 4 Direct Spectrophotometric Analysis Direct spectrophotometric analysis of chlordiazepoxide hydrochloride has not been found to be applicable if any of the known hydrolytic contaminants are present. For material not containing the interfering species, the reported maxima at 245-6 nm and 311-12 nm in acidified 3A alcohol may be used for quantitative measurements.

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