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By W. J. Armitage (auth.), C. Th. Smit Sibinga, P. C. Das, H. T. Meryman (eds.)

The subject matter of this 14th overseas Symposium on Blood Transfusion is heavily on the topic of the paintings and clinical contributions of the Dutch cryobiology pioneer Dr. Herman W. Krijnen of the Dutch crimson pass valuable Laboratory. Dr. Krijnen was once recognized and revered within the nationwide and interna­ tional blood transfusion group as a very efficient scientist and a loved and well known colleague. Dr. Krijnen used to be deliberately honoured with the invitation to open this symposium on cryopreservation and occasional temperature biology in blood transfusion and be the visitor of honour at this occasion. regrettably, Dr. Krijnen unexpectedly died at the first of June 1989. In honour and mem­ ory of Dr. Krijnen this symposium will as a result be devoted to him. because the lOth foreign Symposium on Blood Transfusion in 1985 highlighted the topic of "Future advancements in blood banking", significant alterations have happened within the blood banking global. every one of these adjustments have been pressured upon the Blood Banks via the terror of spreading AIDS via infected donations. This not just ended in the huge­ unfold trying out of blood, but in addition to a extra acceptable counselling of the group and the blood donors in particular. also, virus inacti­ vation recommendations have been brought for these elements derived from a number of donations and meant for a standard transfusion in haemophi­ lia sufferers and others.

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Additional resources for Cryopreservation and low temperature biology in blood transfusion: Proceedings of the Fourteenth International Symposium on Blood Transfusion, Groningen 1989, organised by the Red Cross Blood Bank Groningen-Drenthe

Example text

These results demonstrate the importance of tempering or conditioning plasma to produce an optimal cryoprecipitate. They are also consistant with the findings of Winkelman and Pinnell [26] and Farrugia et al [27] in suggesting that, within a limited range, the conditioning temperature does not influence Factor VIII yield but does affect the amount of protein which is co-precipitated with Factor VIII. Furthermore our results show that the yield of Factor VIII into the cryoprecipitate is dependent upon a controlled temperature rise during this first stage of thawing.

A vIh Hoff plot, recorded after four times freeze-drying; log K is plotted versus liT. The data refer to one specific 31 p resonance (k) in the spectrum of glutamic acid specific tRNA from E. coli. 45 resolved 3I p resonances can be used to monitor the preservation of the tertiary structure after freeze-drying (freezing rate: 40°C/min; cycle: 24 hours). A vlh Hoff plot, based upon 3I p NMR data, is shown in Figure 16: Log K (equilibrium constant) is plotted versus liT (reciprocal absolute temperature).

The individual chromatographic runs have been interchanged with freeze-drying runs to concentrate and preserve the tRNA during ongoing purification. Chromatographic detection and purity determination of individual tRNAs was performed using a specific enzyme radio-isotope assay [9]. 31 p NMRspectra oftRNA p 3I NMRspectra were recorded on a Varian XL-100 spectrometer, operating in the Fourier transform mode at 40,5 MHz. Heteronuclear proton noise decoupling was used to remove the J coupling induced by the ribose protons.

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